Figure 7.

In vitro SnRK1 activity for control and salt-treated leaf and kernel tissue. SnRK1 activity was determined in tissue extracts from control and salt-stressed plants at silking, pollination, and 3 DAP as described by Zhang et al. (2009). Exogenous T6P was added to some samples to determine the fraction of SnRK1 activity sensitive to T6P inhibition (1 mm). Lowercase letters indicate significant difference from controls (P < 0.05; n = 3) as follows: SnRK1 activity higher in kernel than leaf (a); exogenous T6P inhibits SnRK1 activity to a greater extent in kernel tissue (b); SnRK1 activity drops significantly in control kernels after pollination (c); kernel SnRK1 activity increases after pollination in salt-treated plants (d); SnRK1 activity increases significantly in control leaves after pollination (e); and salt treatment results in a significant decrease in SnRK1 activity at 3 DAP (f). Values are means ± sd.