Figure 4.

Induction of CK-responsive gene expression by MeCKs. Two-week-old Arabidopsis wild-type seedlings were treated with iP, 1MeiP, or 2MeiP at the indicated concentrations by vacuum infiltration for 1 h. Expression levels of ARR5 (A) and ARR6 (B) were analyzed by quantitative reverse transcription-PCR. The expression levels were normalized using ACTIN8 (ACT8) as the internal control. Error bars represent the sd of four or five biological replicates. Asterisks indicate statistically significant differences compared with a mock treatment (one-way ANOVA, P < 0.05).