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. 2015 Aug 24;169(2):1275–1291. doi: 10.1104/pp.15.01112

Figure 9.

Figure 9.

Overexpression of SSPP rescues the SARK-induced precocious leaf senescence in Arabidopsis. A, Four-day-old GVG:GUS (G28), GVG:AtSARK (AtS20), 35S:SSPP, and 35S:SSPP/GVG:AtSARK transgenic plants were grown on vertical plates containing either 10 μm DEX or its mock solution for an additional 7 d. Results from one out of three biological replicates are shown. B, Determination of AtSARK and SSPP transcript levels by quantitative RT-PCR. Four-day-old GVG:GUS, GVG:AtSARK, and GVG:AtSARK/35S:SSPP were incubated with either 10 μm DEX or its mock solution for 24 h. The data are displayed as relative expression to the mock-treated control. Three biological replicates with at least three technical repeats were done. Error bars represent sd. C, Overexpression of SSPP effectively reduces the AtSARK-induced expression of senescence-related marker genes in Arabidopsis. Quantitative RT-PCR analysis was used to determine the expression levels of SAG12, NAC1, WRKY6, and SAG113 in 4-d-old GVG:GUS, GVG:AtSARK, 35S:SSPP, and 35S:SSPP/GVG:AtSARK transgenic seedlings treated with either 10 μm DEX or its mock solution for 24 h. TIP41-like was used as an internal control. In all cases, data are shown relative to the mock-treated control. Three biological replicates with at least three technical repeats were done.