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. 2015 Sep 28;10(9):e0139037. doi: 10.1371/journal.pone.0139037

Fig 2. Expression of Activation Markers using flow cytometry assays.

Fig 2

Histograms showing the expression of CD25, CD69, CD38 and HLA-DR from gated CD3+CD4+ cell populations from PBMCs are shown in the upper row. Histograms indicating the expression of CD25, CD69 from gated CD3+CD4+ cell populations from purified T CD4+ cells appear in the lower row. X axis indicates the fluoresce intensity of a specific marker and Y axis indicates the percentage of cells in the population expressing this specific marker. Graphics under each histogram represent the MFI (Median of Fluorescence Intensity) of each activation marker in the gated CD3+CD4+ cell populations. Results are shown as mean values ± SD and are representative from three independent experiments. Statistical significance was assessed by the Two-tailed Student’s t-test yielding * when p < 0.05; and **, when p < 0.005. Gray area: unstained control cells, green/blue line: non-stimulated cells, red line: stimulated cells.