Table 5. Types of nucleotide substitutions in R5 and X4 pseudotyped virus infections.
Mutated positions and the frequency of replaced nucleotides in the proviral population per experimental conditions.
| Mutated position | Non-stimulated CD4 + cells/ X4 HIV-1 | Non-stimulated CD4 + cells/ R5 HIV-1 | Stimulated PBMC/ X4 HIV-1 | Non-stimulated PBMC/ X4 HIV-1 |
|---|---|---|---|---|
| 3242 | G→T (55.1%) | |||
| 6438 | C→T (98.5%) | |||
| 6780 | A→C (50.9%) | A→C (51.6%) | A→C (50.7%) | |
| 7766 | A→T (80.6%) | A→T (73.4%) | A→T (70.5%) | |
| 7819 | A→G (99.4%) | A→G (99.1%) | A→G (98.4%) | |
| 7842 | T→A (93.9%) | T→A (94.7%) | T→A (96.0%) | |
| 7864 | A→G (99.5%) | A→G (99.1%) | A→G (99.2%) | |
| 7876 | C→T (54.2%) | C→T (50.0%) | C→T (54.3%) | |
| 7952 | G→A (99.8%) | G→A (99.8%) | G→A (99.9%) | |
| 7977 | G→A (99.7%) | G→A (99.7%) | G→A (99.7%) | |
| 7993 | A→G (99.8%) | A→G (99.8%) | A→G (99.8%) | |
| 8023 | C→T (99.8%) | C→T (99.8%) | C→T (99.8%) | |
| 8087 | A→C (99.8%) | A→C (99.9%) | A→C (99.8%) | |
| 8091 | T→C (99.8%) | T→C (99.8%) | T→C (99.8%) | |
| 8382 | T→C (98.5%) | T→C (99.5%) | T→C (99.8%) | |
| 8473 | A→G (99.8%) | A→G (99.7%) | A→G (99.8%) | |
| 8652 | A→G (99.8%) | A→G (99.8%) | A→G (99.8%) | |
| 8711 | T→G (99.9%) | T→G (99.8%) | T→G (99.8%) | |
| 8718 | C→G (99.9%) | C→G (99.9%) | C→G (99.9%) | |
| 8724 | A→G (99.8%) | A→G (99.8%) | A→G (99.8%) | |
| 8829 | G→A (99.8%) | G→A (99.7%) | G→A (99.8%) | |
| 8871 | G→A (99.7%) | G→A (99.6%) | G→A (99.7%) | |
| 8884 | T→C (99.8%) | T→C (99.8%) | T→C (99.8%) | |
| 8897 | A→G (99.8%) | A→G (99.8%) | A→G (99.8%) | |
| 8938 | A→C (99.8%) | A→C (99.8%) | A→C (99.8%) | |
| 8978 | A→G (99.4%) | A→G (99.8%) | A→G (99.8%) |
Underlined = transversions, italics = transitions. Infections of stimulated T CD4 + cells and stimulated and non- stimulated PBMCs by R5 pseudotypes did not result in changes at the most probable nucleotide.