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. Author manuscript; available in PMC: 2016 Jun 16.
Published in final edited form as: Langmuir. 2015 Jun 4;31(23):6554–6562. doi: 10.1021/acs.langmuir.5b00829

Figure 5.

Figure 5

Comparison of mismatch discrimination recorded with the two-electrode platform using methylene blue vs. Nile blue redox reporters. (a) Electrochemical readout recorded at a secondary electrode during the electrocatalytic reduction of 300 μM Fe(CN)63− in the presence of 4 μM MB at primary electrodes modified with well-matched DNA duplexes (blue), and mismatched DNA duplexes (red). (b) The same assay, but with Nile blue covalently bound to the DNA probe sequences substituting for MB. Percent signal changes were calculated from the amperometry data recorded 90 seconds after initiation of the electrochemical readout.