Extended Data Figure 10. Representative images and distribution of individual samples for projection-based viral-genetic labeling experiments.
a, Representative images from sagittal sections of TC+ LC-NE axons in 8 brain regions indicated at the top of each column (the last column shows cell bodies for LC-NE neurons) resulting from CAV injections at four projection sites indicated on the left (top four rows), or AAV-FLExloxP-TC injection at the LC of Dbh-Cre animals (bottom row). All TC+ processes were confirmed to contain norepinephrine transporter (NET, an NE neuron marker) by anti-NET immunostaining (not shown; see Fig. 4 inset). b, The normalized fraction of TC+ LC-NE axons for individual experiments for five conditions are color coded on the top right. Filled symbols represent experiments where Dbh-Cre mice were used along with CAV-FLExloxP-Flp; open symbols represent experiments where wt mice were used along with CAV-Cre. The distribution of individual samples with regards to the fraction of TC+ axons observed at output sites was similar between wt and Dbh-Cre mice. Collectively, the samples for each condition were averaged to quantify the normalized fraction of TC+ LC-NE axons in each brain region as reported in Fig. 4d. Scale, 50 μm. Error bars, s.e.m. Abbreviations: AC, auditory cortex; CC, cingulate cortex; Cb, cerebellum; Hi, hippocampus; Hy, hypothalamus; LC, locus coeruleus; Me, medulla; OB, olfactory bulb; SC, somatosensory cortex.