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. Author manuscript; available in PMC: 2016 Feb 6.
Published in final edited form as: Nature. 2015 Jul 1;524(7563):88–92. doi: 10.1038/nature14600

Figure 2. Presynaptic input to locus coeruleus norepinephrine (LC-NE) neurons revealed by rabies-mediated trans-synaptic tracing.

Figure 2

a, Strategy for trans-synaptic tracing of input to LC-NE neurons. b, Coronal section of a mouse brain at the LC (dotted square) stained with DAPI (blue). A region within the square is magnified in the inset. LC-NE starter cells (yellow) can be distinguished from cells receiving only TC from AAV (red) or only GFP from RVdG (green) at the injection site. c–g, Coronal sections showing representative input neurons in diverse brain regions. h, Sagittal section of the cerebellum showing trans-synaptically labeled Purkinje cells. Images in b–h were derived from larger composites. i, Schematic summary of brain regions that provide the largest average fractional inputs to LC-NE neurons (n=9). Scale, 1 mm (b), 50 μm (b, inset; c–h). Abbreviations: BNST, bed nucleus of the stria terminalis; CeA, central amygdala; DCN, deep cerebellar nuclei; IRN, intermediate reticular nucleus; LH, lateral hypothalamus; LRN, lateral reticular nucleus; LC, locus coeruleus; MRN, midbrain reticular nucleus; PVH, paraventricular hypothalamic nucleus; PGRN/GRN, paragigantocellular/gigantocelluar nucleus; PAG, periaqueductal gray; PRN, pontine reticular nucleus; POA, preoptic area; PC, Purkinje cells; SVN, spinal vestibular nucleus; SuC, superior colliculus; ZI, zona incerta.