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. Author manuscript; available in PMC: 2016 Feb 3.
Published in final edited form as: Biochemistry. 2015 Jan 21;54(4):962–973. doi: 10.1021/bi501520w

Figure 5.

Figure 5

Electrochemistry of DNA glycosylases on DNA-modified electrodes. Both single and multiplexed DNA-modified gold electrodes have been used to study the electrochemical characteristics of DNA glycosylases containing [4Fe-4S] clusters. In these experiments, the protein is bound to the DNA, which is attached to the gold electrode surface, and the [4Fe-4S] cluster is reduced via DNA CT (left). Cyclic voltammetry has been used to determine that the midpoint redox potential of these enzymes is around 80 mV vs. NHE.20,22,48 and to establish that EndoIII Y82A is deficient in its ability to perform DNA-mediated CT (right). The intensity of the electrochemical signal of EndoIII Y82A is much lower than that of WT EndoIII, depicted by the red and blue arrows, respectively, in the illustration (left). The redox-active [4Fe-4S] clusters in the proteins are signified here by clusters of two orange and two yellow spheres.