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. 2015 Sep 28;8:484. doi: 10.1186/s13104-015-1405-4

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© Michaelis et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — ABCG2 depletion using a second lentiviral vector (LeGO-X-GFP2) with dsRedExpress as marker encoding an shRNA targeting the bicistronic ABCG2-IRES-eGFP mRNA of the first vector (LeGO-iG2-ABCG2) thereby depleting eGFP and ABCG2 expression. a Fluorescence pictures indicating dsRedExpress (encoded as marker by LeGO-X-GFP2) and eGFP fluorescence in UKF-NB-3 cells transduced with LeGO-iG2-ABCG2 (UKF-NB-3^ABCG2) or LeGO-iG2-ABCG2 and LeGO-X-GFP2 (UKF-NB-3^ABCG2-XGFP2). b ABCG2 levels in UKF-NB-3 cells, UKF-NB-3 cells transduced with the empty LeGO-iG2 vector (UKF-NB-3^iG2), UKF-NB-3^ABCG2 cells, or UKF-NB-3^ABCG2-XGFP2 cells as determined by flow cytometry and expressed as relative fluorescence units (rfu). *P < 0.05 relative to UKF-NB-3