Fig. 2.
Caffeine-induced diuretic and natriuretic response is independent of NHE3 abundance and phosphorylation at serine 552 (pS552) or serine 605 (pS605) in renal membranes. Kidneys of control (NHE3loxlox) mice were harvested 1 h after application of vehicle (water, 1% of body wt) or caffeine (45 mg/kg body wt by oral gavage). Data are expressed relative to the mean of the vehicle-treated control group, which was set as 100%. Black lines indicate where the original blots were spliced. n = 5/group.