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In the mouse kidney ClC-K2 is localized to the medullary thick ascending limb. Representative images of diaminobenzidine-stained kidneys show ClC-K2 in the thick ascending limb (A), and caffeine application did not clearly alter this staining pattern (B). mTAL, medullary thick ascending limb; n = 6/condition. Scale bar = 25 μm.
