Table 5.

qRT-PCR validation of selected miRNA-mRNA pairs

miRNA/mRNA	miR-181b, array	miR-181b, qPCR	Pdgfra, array	Pdgfra, qPCR
B6129 6 h/saline	−1.25	−1.47 ± 0.83	1.63	−2.02 ± 0.50
B6129 20 h/saline	1.00	−2.38 ± 1.14	1.19	−2.02 ± 0.78
E2F1 6 h/saline	−2.00	−2.27 ± 0.48	0.96	1.72 ± 0.46
E2F1 20 h/saline	−2.22	−3.45 ± 0.88	2.38	1.87 ± 0.40

miRNA relative expression levels were assessed using Taqman assays, and mRNA levels were measured using SYBRGreen. Reported are means with SD of 4-6 animals. Bold indicates validated mRNA-miRNA anti-correlated expression relationship.