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. 2015 Sep 28;10(9):e0138760. doi: 10.1371/journal.pone.0138760

Fig 3. Time-lapse analyses of plaque phenotypes from VACV WR and IHD-J strains demonstrate contribution of cell-free virus to spreading.

Fig 3

(A) Still micrographs of representative plaque phenotypes from VACV-IHD-J-E/L-GFP and VACV-WR-GFP in either gelled or liquid medium. (B) Example of VACV-WR-E/L-GFP live microscopy plaques (24 h pi) analyzed by Plaque2.0 software. Here superimposed on the micrograph from the GFP signal, green colored pixels designate foreground pixels detected by thresholding, colored line designates plaque borders and red spots highlight local intensity maxima. This procedure allows detection of adjacent plaques. (C) The relative plaque area normalized to the plaque area from gelled medium was plotted as a function of time. Note that VACV-IHD-J plaques occupy a larger area than VACV-WR at late stages of infection owing to cell-free EEV. Results represent averages from over 50 plaques for each condition from 8 technical replicas.