Skip to main content
. 2015 Feb 2;26(10):2361–2377. doi: 10.1681/ASN.2014040405

Figure 4.

Figure 4.

Prenatal nephrin knockdown causes massive proteinuria. Prenatal DOX feeding for nephrin knockdown during development. (A) A schematic of the breeding scheme with the genotype of parents and pups specified. Inseminated female was fed DOX on day E7. The observed percentage of pups with a given genotype indicated within parentheses. (B) Urinary albumin excretion of pups with and without nephrin knockdown, sh3703 and Luci.1309, respectively. (C) Western blots of kidney lysates of sh3703 and Luci.1309 pups. Each lane is a lysate from a different mice. (D) Period acid-Schiff–stained kidney sections of Luci.1309 and sh3703 pups with prenatal DOX feeding. Yellow arrows in part c, dilated Bowman capule with collapsed glomeruli. Green arrow in part d, protein resorption droplets within tubular cells. Asterisk marks a tubular cast within the tubular lumen. Green arrowheads in part e, visceral epithelial glomerular cells. Green arrow in part e, segment of glomerular basement membrane without podocytes. Double-headed green arrow in part e, dilated capillary loop. Error bars, SEM.