Table 3.

Primers for RT-PCR

Gene	Forward	Reverse
Gapdh	5′-GCCATCAACGACCCCTTCAT	5′-ATGATGACCCGTTTGGCTCC
Nphs1	5′-GTGCCCTGAAGGACCCTACT	5′-CCTGTGGATCCCTTTGACAT
Nphs2	5′-CTTGGCACATCGATCCCTCA	5′-CGCACTTTGGCCTGTCTTTG
Cldn3	5′-GTCCGTCAGTTTTCGAAGGG	5′-AGGATGGACACCACGATGAG
Synpo	5′-CTTTGGGGAAGAGGCCGATTG	5′-GTTTTCGGTGAAGCTTGTGC
Neph1	5′-TCACAAGCAGGGCTTTAGGA	5′-CTGGCTGAAGCGAGTCTGAG
Neph3	5′-ACATTTCCGGAACCCAGGAG	5′-CCACAATCCGGACAGTAGGC
CD2AP	5′-TGGTGGAGTGGAACCCTGAA	5′-GTGAGGTAGGGCCAGTCAAA
P-cadherin	5′-GATTTTGAGGCTCAGGACCA	5′-GACCTTGGAAGGTGGAACAA
Inf2	5′-CACCATCTCTCGGCTGTACC	5′-CGAGTAGTTGACCACCGAGG
Fat1	5′-CCAGGGAAGTCCGTTCACAA	5′-TTGACTCTGGGTGGATTGCC
Actn4	5′-TTCGACAACAAGCACACCAA	5′-CCAGTGCCCCGCCAT
Wt1	5′-GAGAGCCAGCCTACCATCC	5′-GGGTCCTCGTGTTTGAAGGAA
JAM-A	5′-CGCGTCGGGATTGTAACTGT	5′-CACCGAACCCTTGCCTTGTA
Occludin	5′-AGGTGAGCACCTTGGGATTC	5′-TTCAAAAGGCCTCACGGACA
Cingulin	5′-TGTGAATCCGGGAGCACTGA	5′-TAGCACCCTCTGGCTCTGTA
ZO-1	5′-TAGCACGGACAGTAGACACA	5′-ATGGAAGTTGGGGTTCATAG