Figure 2.

Drosha knockdown does not promote changes in EV surface markers and up-take by mTEC. (A) Representative FACS analyses of the expression of classic MSC markers (CD29, CD44, CD105, CD73, CD90, and HLA class I) by EV-CTRL and EV-Dsh. Dotted lines indicate the isotype controls. Three different EV preparations were tested with similar results. (B) Representative micrograph of incorporation of EV-CTRL and EV-Dsh by mTECs after 24 hours of incubation. EVs were collected from MSCs double stained with Vybrant Dil (red) and SYTO-RNA (which labels the RNA; green). Three experiments were performed with similar results. Original magnification, ×200; enlarged image magnification, ×630.