Figure 1.

SPLX exacerbates kidney IRI. Mice underwent SPLX or sham surgery 7 days before a subIRI (24 minutes of ischemia, 24 hours of reperfusion), which was not sufficient alone to cause AKI. Prior SPLX exacerbated subthreshold injury and resulted in elevated plasma creatinine (A) and robust ATN (B) (scored from hematoxylin and eosin samples) (C). ATN, acute tubular necrosis; SPLX, splenectomy; subIRI, subthreshold ischemia-reperfusion injury. n=6–13. *P≤0.001. Scale bar is 100 μm in the main panel and 50 μm in the inset.