Table 2.
Detection of cross-reactivity between calpain-like proteins from Angomonas deanei wild type and aposymbiotic strains and anti-calpain antibodies by flow cytometric analysis
| Anti-calpain Antibody | Non-Permeabilized Parasites | Triton X-100-Permeabilized Parasites | ||||||
|---|---|---|---|---|---|---|---|---|
| Wild Type Strain | Aposymbiotic Strain | Wild Type Strain | Aposymbiotic Strain | |||||
| % FCa | MFIa | % FC | MFI | % FC | MFI | % FC | MFI | |
| Anti-Dm-calpain | 6.3 ± 0.4 | 4.1 ± 0.2 | 5.3 ± 0.5 | 4.6 ± 0.5 | 80.0 ± 3.9 | 44.4 ± 6.8** | 81.1 ± 8.9 | 19.7 ± 3.2** |
| Anti-CAP5.5 | 8.2 ± 0.8 | 4.7 ± 0.3 | 6.0 ± 1.0 | 4.3 ± 0.5 | 22.1 ± 1.8* | 10.1 ± 0.2 | 44.2 ± 9.9* | 13.5 ± 0.6 |
| Anti-CDPIIb | 6.1 ± 0.9 | 2.8 ± 0.2 | 4.4 ± 1.2 | 2.4 ± 0.2 | 39.8 ± 4.0 | 16.9 ± 1.5 | 39.9 ± 5.4 | 18.6 ± 0.4 |
a % FC, percentage of fluorescent cells; MFI, mean of fluorescence intensity. Symbols (*,**) denote significant different concerning either the % FC (*) or MFI (**) between wild type and aposymbiotic strains (P <0.05). Additionally, both the percentage of antibody-labeled cells and the MFI values for all tested antibodies were significantly different between non-permeabilized and Triton X-100-permeabilized parasite cells. Representative data of the analysis of 10,000 cells from 3 experiments are shown