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. 2015 Sep 29;15:188. doi: 10.1186/s12866-015-0519-0

Table 2.

Detection of cross-reactivity between calpain-like proteins from Angomonas deanei wild type and aposymbiotic strains and anti-calpain antibodies by flow cytometric analysis

Anti-calpain Antibody Non-Permeabilized Parasites Triton X-100-Permeabilized Parasites
Wild Type Strain Aposymbiotic Strain Wild Type Strain Aposymbiotic Strain
% FCa MFIa % FC MFI % FC MFI % FC MFI
Anti-Dm-calpain 6.3 ± 0.4 4.1 ± 0.2 5.3 ± 0.5 4.6 ± 0.5 80.0 ± 3.9 44.4 ± 6.8** 81.1 ± 8.9 19.7 ± 3.2**
Anti-CAP5.5 8.2 ± 0.8 4.7 ± 0.3 6.0 ± 1.0 4.3 ± 0.5 22.1 ± 1.8* 10.1 ± 0.2 44.2 ± 9.9* 13.5 ± 0.6
Anti-CDPIIb 6.1 ± 0.9 2.8 ± 0.2 4.4 ± 1.2 2.4 ± 0.2 39.8 ± 4.0 16.9 ± 1.5 39.9 ± 5.4 18.6 ± 0.4

a % FC, percentage of fluorescent cells; MFI, mean of fluorescence intensity. Symbols (*,**) denote significant different concerning either the % FC (*) or MFI (**) between wild type and aposymbiotic strains (P <0.05). Additionally, both the percentage of antibody-labeled cells and the MFI values for all tested antibodies were significantly different between non-permeabilized and Triton X-100-permeabilized parasite cells. Representative data of the analysis of 10,000 cells from 3 experiments are shown