Table 2.
Stability of plasmids and mini-Tn7 insertions in different strains
| Strain | Free-living stabilitya | Stability in symbiosisb |
|---|---|---|
| Ret pJB3Tc19 | 8,6 % | 98 % |
| Ret pJBpleD* | 0.6 % | 0 % |
| Ret Tn7Km | 100 % | 100 % |
| Ret Tn7pleD*Km | 100 % | 90 % |
| Ret Tn7Tc | 100 % | n.d. |
| Ret Tn7pleD*Tc | 100 % | n.d. |
| Rle pJB3Tc19 | 73,5 % | 86 % |
| Rle pJBpleD* | 67,1 % | 70 % |
| Rle Tn7Km | 100 % | 100 % |
| RleTn7pleD*Km | 100 % | 100 % |
| Rle Tn7Tc | 100 % | n.d. |
| Rle Tn7pleD*Tc | n.d | n.d. |
| Sme pJB3Tc19 | 2,8 % | 51 % |
| Sme pJBpleD* | 3,1 % | 22 % |
| Sme Tn7Km | 100 % | 100 % |
| Sme Tn7pleD*Km | 100 % | 91 % |
| Sme Tn7Tc | 100 % | n.d. |
| Sme Tn7pleD*Tc | 100 % | n.d. |
| Pto pJB3Tc19 | 100 % | n.d. |
| Pto pJBpleD* | 76,8 % | n.d. |
| Pto Tn7Km | 100 % | n.d. |
| Pto Tn7pleD*Km | 100 % | n.d. |
| Pto Tn7Tc | 100 % | n.d. |
| Pto Tn7pleD*Tc | 100 % | n.d. |
aBacteria were grown in rich medium without antibiotics for at least 100 generations; the stability was determined as the ratio of CFU recovered on selective medium out of the total CFU obtained in nonselective medium
bPercentage of nodules containing bacteria that kept antibiotic resistance (for more details see supporting information)
n.d., not determined