Table 3.
Efficacy of the lacI q-IPTG system to modulate pleD* expression
| Strain | Relative pleD* expression1 | Intracellular c-di-GMP 2 | EPS production3 | |||
|---|---|---|---|---|---|---|
| IPTG - | IPTG + | IPTG - | IPTG + | IPTG - | IPTG + | |
| Sme Tn7pleD*Km pBBR1MCS5 | 15,11 ± 1,98 | n.d. | 317,16 ± 13,29 | n.d. | 2,66 × 106 ± 6,9 × 104 | 2,64 × 106 ± 7,34 × 104 |
| Sme Tn7pleD*Km pBBRlacIq | 1,00 ± 0,26 | 6,08 ± 1,06 | 55,10 ± 16,27 | 152,05 ± 8,50 | 3,51 × 105 ± 1,19 × 104 | 2,23 × 106 ± 6,36 × 104 |
| Sme Tn7Km | - | - | - | n.d. | 6,20 × 104 ± 5,54 × 103 | 7,39 × 104 ± 3,44 × 103 |
1Relative expression (fold change) to Sme Tn7pleD*Km pBBRlacIq strain without IPTG (repression state) by qRT-PCR. In all strains pleD* expression was normalized with to 16S rRNA levels
2pmol of c-di-GMP/mg of total protein. The c-di-GMP levels of Sme Tn7Km is under the technical limit of detection
3CF-derived Fluorescence (arbitrary units)
n.d., not determined