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. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: Breast Cancer Res Treat. 2015 Apr 23;151(2):281–294. doi: 10.1007/s10549-015-3376-5

Fig. 1.

Fig. 1

Breast cancer cell lines with BCSC properties. a Mammosphere formation assay in MCF7, SK-BR-3, MDA-MB-231, and HCC1937 cells. Images show representative mammospheres in each cell line at day 5 of incubation. Original magnification: ×200. b ALDEFLUOR assay in breast cancer cell lines (HCC1937, MDA-MB-231, MCF7, and SK-BR-3). The upper panels show the negative controls (cells treated with DEAB, a specific inhibitor of ALDH1). Based on the negative control, the gated area was assigned as ALDH1-positive. Percentages of ALDH1-positive cell populations of each cell lines are shown in the lower panels