Fig. 6.

Effect of AZD8055 (AZD) on mTOR-S6K axis in MK-2206-resistant sublines. a–d After 1 h serum starvation, indicated cell lines were incubated in RPMI1640 + 10 % FBS with/without MK-2206 (5 μM) or AZD (50 nM). Phosphorylation of mTOR and S6K were detected by western blot at 1.5 and 12 h, so was Actin. e Effect of U0126 on NB-19-MK cell line compared with NB-19. Indicated cells were treated with U0126 at indicated concentrations in RPMI1640 + 10 % FBS, with/without MK-2206 (5 μM). Cell growth was evaluated as cell number at 72 h, and result was repeated three times. Data are expressed as the mean (±SD). f NB-19 and NB-19-MK were incubated in RPMI1640 + 10 % FBS with/without MK-2206 (5 μM) or U0126 (5 μM). Phosphorylation of ERK, AKT, and S6K were detected by western blot at 1.5 and 12 h, so was Actin