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. 2015 Sep 29;10(9):e0138107. doi: 10.1371/journal.pone.0138107

Table 1. Primers used for RT PCR of mRNA of specific genes expressed in astrocytes and microglia.

Astrocytes markers
Glial high affinity glutamate transporter, member 2 (Slc1a2) FWD: 5'-agtgtgtctatgccgcacac-3'
REV: 5'-ggctgagaatcgggtcatta-3'
Aldehyde dehydrogenase 1 family, member L1 (Aldh1l1) FWD: 5'-gcaccttttggaggattcaa-3'
REV: 5'-ggcaacagggtacaggagaa-3'
Fibroblast growth factor receptor 3 (Fgfr3) FWD: 5'-cgttgtagagagggctggac-3'
REV: 5'-gaggcttgccacataagagc-3'
Microglia markers
Allograft inflammatory factor 1 (Aif1) FWD: 5'-aatgatgctgggcaagagat-3'
REV: 5'-cctccaattagggcaactca-3'
Integrin, alpha M (Itgam) FWD: 5'-cgtttgtgaaggctcagaca-3'
REV: 5'-gtcgtttgaaaaagccaagc-3'
Integrin, beta 2 (Itgb2) FWD: 5'-agtcccagtggaacaacgac-3'
REV: 5'-ggaggggtctcctctgactc-3'
Chemokine (C-X3-C motif) receptor 1 (Cx3cr1) FWD: 5'-gcctcaacccctttatctacg-3'
REV: 5'-tgctcctctgggactctgtt-3'
Oligodendrocytes markers
Myelin basic protein (Mbp) FWD: 5'-ccgaggagagtgtgggttta-3'
REV: 5'-ctggagggtttgtttctgga-3'
Myelin-associated oligodendrocyte basic protein (Mobp) FWD: 5'-caggaccacacgatttctt-3'
REV: 5'-ggaggtcagagcacaagagg-3'
Neuronal markers
Syntaxin 1A (Stx1a) FWD: 5'-gctctgaaaagccaggtgtc-3'
REV: 5'-cagctggctgctgaatactg-3'
Neurofilament, medium polypeptide (Nefm) FWD: 5'-agtggtggtgaccaagaagg-3'
REV: 5'-caccagcttctcctcaaagg-3'