Fig. 2.

Expression and promoter methylation analyses of TAGLN gene in BC cell lines. a Box-plot analysis based on qRT-PCR results showing strong downregulation of TAGLN expression in BC cell lines compared to NTB cell lines. Log₂ expression levels relative to GAPDH reference gene are shown. Horizontal dashed lines represent median values. Error bars: standard error of means (SEM). **P < 0.01; Mann–Whitney Test. b qRT-PCR analysis of AZA-treated BC and NTB cell lines indicating high levels of induction in TAGLN expression levels. Values represent the log₂ ratios of expression levels in AZA-treated cell lines to those in their DMSO-treated cells. c QUMA software analyses based on bisulfite sequencing of BC and NTB cell lines showing hypermethylation in TAGLN promoter in BC cells. Percent values represent the average methylation of 5 clones for each cell line. Full circles: methylated; Empty circles: unmethylated. Sequence numbers of CpGs are shown above circles. d QUMA analysis showing significantly higher methylation in BC cells compared to NTB; Mann–Whitney test. Methylated DNA ratio: black area; unmethylated ratio: white area in the pie chart. e Scatter plot showing the spearman correlation of TAGLN methylation status with log₂(expression) levels in BC and NTB cell lines