Fig. 6.
Effect of TAGLN overexpression and silencing on breast cell lines. a Western blot analysis of TAGLN in breast carcinoma and non-tumorigenic breast cell lines. b–c Western blot shows TAGLN silencing in MCF10A (b) and MCF12A (c) cells (upper panels). 2D colony formation assays (middle and lower panels) show increased colony formation in TAGLN silenced (siTAGLN) MCF10A (b) and MCF12A (c) cells compared to controls (siCTRL). d–e Western blot analyses of TAGLN overexpression and subsequent colony formation assays in MDA-MB-361 (d, upper panel), and MDA-MB-157 (e, left panel) cell clones. Overexpression of ectopic TAGLN (TAGLNvec) and levels of endogenous TAGLN (TAGLNint) were determined with TAGLN specific antibody in clones. TAGLN overexpression caused higher cell proliferation in MDA-MB-361 cells (e), while did not affect MDA-MB-157 cells (f). Each assay was conducted at least in quadruplicates. Two sample t test was used for comparisons. Error bars represent standard errors. **P < 0.01