Skip to main content
. 2015 Sep 29;10(9):e0139620. doi: 10.1371/journal.pone.0139620

Fig 4. γ-glutamyl transpeptidase (GGT) stimulates osteoclastogenesis-related gene expression in ST2 cells.

Fig 4

(A) (a) ST2 cells were treated with 100 ng/ml of recombinant human GGT (rhGGT) for 2, 4, 12 and 24 h. VEGF-A, MCP-1, LIX, IFN-γ, TNF-α, IL-1β, RANKL and OPG mRNA expression was analyzed by RT-PCR. ST2 cells were treated with 100 ng/ml of recombinant human GGT (rhGGT) for 1 and 2d. Quantificaation of TNF-α (b) and MCP-1 (c) was performed by realtime PCR. (B) 100 ng/ml of AGT3 was added 2 h before 100 ng/ml of rhGGT treatment. After 2 h of incubation with rhGGT, TNF-α mRNA expression was examined by RT-PCR. (C) Bone marrow cells were cultured with conditioned medium of GGT-stimulated ST2 with or without TNF-α neutralizing antibody (TNF-α Ab). The cell culture stained with TRAP solution.