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. 2015 Sep 29;10(9):e0139620. doi: 10.1371/journal.pone.0139620

Fig 5. γ-glutamyl transpeptidase (rhGGT) inhibits osteoblastic differentiation of osteoblasts in vitro.

Fig 5

(A) Alkaline phosphatase (ALP) activity in MC3T3-E1 cells after 6d of incubation with rhGGT (10, 100 and 500 ng/ml). (B) Mineralization-related gene expression in MC3T3-E1 cells at 12 and 24 h after incubation with rhGGT (100 ng/ml). (C) Primary osteoblasts (OBs) were cultured in OB differentiation media for 2 w with rhGGT (10, 100, and 500 ng/ml); Alizarin red S staining is shown for each condition; (D) Quantification of stained Alizarin red.