Table 1. PCR primers and probe used in this study.
| Gene | Forward | Revers |
|---|---|---|
| Mouse | ||
| ALP | 5’-TCCCTACCGACCCTGTTGTGA-3’ | 5’-TGGACCTCTCCCTTGAGTGT -3’ |
| BSP | 5’-GTACCGGCCACGCTACTTTCT-3’ | 5’-GTTGACCGCCAGCTCGTTTT-3’ |
| TNF-α | 5'-ATGAGCACAGAAAGCATGATC-3' | 5'-TACAGGCTTGTCACTCGAATT-3' |
| IL-1β | 5’-AGAGAGCCTGTCTTTTCCTCCTTG-3’ | 5’-GCTTCAATGAAAGACCTCAGTGCAG-3’ |
| VEGF-A | 5'-CCT CCG AAA CCA TGA ACT TTC TGC TC-3' | 5'-CAG CCT GGC TCA CCG CCT TGG CTT-3' |
| LIX | 5'-GAAAGCTAAGCGGAATGCAC-3' | 5'-GGGACAATGGTTTCCCTTTT-3' |
| MCP-1 | 5'-AGGTCCCTGTCATGCTTCTG-3' | 5'-TCTGGACCCATTCCTTCTTG-3' |
| IFN-γ | 5'-TTTGAGGTCAACAACCCACA-3' | 5'-CGCAATCACAGTCTTGGCTA-3' |
| RANKL | 5'-CGCTCTGTTCCTGTACTTTCGAGCG-3' | 5'-TCGTGCTCCCTCCTTTCATCAGGTT-3' |
| OPG | 5'-CAGAGACTAATAGATCAAAGGCAGG-3' | 5'-ATGAAGTCTCACCTGAGAAGAACC-3' |
| 18S | 5’-CGCTATCTGACTCGCTG-3’ | 5’-GGAAGGTTCTAGTCAGG-3’ |
Quantitative real time PT-PCR was performed in the Applied Biosystem StepOnePlus™ (Appleid Biosystems), using TaqMan® Fast Advanced Master Mix (Appleid Biosystems) and specific primers and probe for TNF-α (Forward: CCAAATGGCCTCCCTCTCAT, Reverse; GCTACAGGCTTGTCACTCGAATT, Probe; 5’-FAM-CCCAGACCCTCACACACTCAGATCATCTTC-TAMRA-3’) and MCP-1 (Forward: GCTGGAGAGCTACAAGAGGATCAC, Reverse; TGGTTCCGATCCAGGTTTTTA Probe; 5’-FAM-CAGCAGGTGTCCCAAAGAAGCTGTAGTTTTT-TAMRA-3’). Reaction product was quantified with 18S (X03205.1 Eukaryotic 18S rRNA; Appleid Biosystems) as the reference gene.