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. 2015 Aug 21;4(3):406–426. doi: 10.3390/cells4030406

Figure 2.

Figure 2

Nucleocytoplasmic shuttling of the Alp7-Alp14 complex in fission yeast. (A) Architectures of Alp7/TACC and Alp14/TOG. Alp7 has a nuclear localization signal (NLS) in its N-terminal half, whereas Alp7 interacts with Alp14 through its C-terminal end. Alp14 has a nuclear export signal (NES) in its middle region. Asterisks show the cyclin-dependent kinase (CDK) phosphorylation sites in Alp7. (B) The export of the Alp7-Alp14 complex. The NES in Alp14 plays a role in the nuclear export of the complex. (C) How the nuclear import of Alp7-Alp14 is modulated through the cell cycle. Left: During interphase, Alp7-Alp14 shuttles between the nucleus and the cytoplasm through the association of the NLS in Alp7 and importin-α/Cut15. Right: During mitosis, CDK phosphorylates Alp7 to accelerate the import activity of the complex and to promote spindle assembly. (D) Localization of Alp7-Alp14 at the onset of meiosis I. Alp7-Alp14 localizes to kinetochores (KT) even before microtubules that emanated from spindle pole bodies (SPBs) attach to the kinetochores.