Table 1.
Total protein and enzyme activities in T98G and U87 cells during incubation in cell culture medium. The data are presented as mean ± SEM. Statistically significant differences (p < 0.05) between 5 h and 24 h (a—T98G 5 h vs. T98G 24 h; b—U87 5 h vs. U87 24 h) or between cells at the same growth time (c—T98G 5 h vs. U87 5 h; d—T98G 24 h vs. U87 24 h) were determined by one-way ANOVA with Tukey’s Multiple Comparison Test. Statistically significant up-regulation of the enzymes in each cell line during the incubation is shown in bold.
| Cell Line | T98G | U87 | ||
|---|---|---|---|---|
| Time in Medium | 5 h | 24 h | 5 h | 24 h |
| Protein, mg/mL (c,d) | 1.73 ± 0.08 | 1.81 ± 0.00 | 1.33 ± 0.01 | 1.34 ± 0.00 |
| Activity, μmol/(min∙mg) | ||||
| OGDH (b,d) | 0.05 ± 0.01 | 0.03 ± 0.00 | 0.07 ± 0.02 | 0.16 ± 0.01 |
| GDH (b) | 0.016 ± 0.003 | 0.032 ± 0.003 | 0.030 ± 0.002 | 0.052 ± 0.012 |
| MDH (a,b,c,d) | 7.8 ± 0.4 | 13.3 ± 0.3 | 10.6 ± 0.6 | 18.7 ± 0.9 |
| Malic (a,d) | 0.09 ± 0.01 | 0.57 ± 0.01 | 0.06 ± 0.01 | 0.11 ± 0.02 |