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. 2015 Sep 11;4(3):500–519. doi: 10.3390/cells4030500

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

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Immunofluorescence analysis of olfactory cilia in the mouse olfactory epithelium. (A–C) Confocal laser scanning micrographs of a cryostat section through adult mouse olfactory epithelium double labeled with antibodies against Centrin 3 (green) and polyglutamylated tubulin (red); (A,B) Centrin 3 and polyglutamylated tubulin are localized in the ciliary region of the olfactory sensory neurons (OSNs); (C) Merge combined with a DAPI nuclear staining (blue); (D–F) Higher magnification views of the ciliary region; (D) Centrin 3 is preferentially localized at the basal body, the centriole and the transition zone of cilia in the olfactory knobs (OK) of receptor neurons; (E) Polyglutamylated tubulin labels the axoneme of olfactory cilia; (F) The merge shows a partial colocalization of Centrin 3 and polyglutamylated tubulin at the transition zone of the olfactory receptor neurons. ML: mucus layer. Scale bars: 10 µm (C for A–C), 1 µm (F for D–F).