Fig. 1.

Social isolation during adolescence alters adult social interaction and NSF. a Mice tested 4 weeks after isolation from weaning spent the same distance in the open-field test. b Isolated mice spent less time in the interaction zone when there was a target mouse than control mice. No difference was observed in the time in the interaction zone when there was no target mouse or corner zone. c, d In the novelty-suppressed feeding test, the latency to explore and bite the food was increased, but no difference in food intake amount was observed. Vertical bars represent the mean ± SEM. Asterisks indicate significant differences from the relevant controls (n = 10/group, p < 0.05, **p < 0.01, two-tailed t test)