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. 2015 Sep 29;15:192. doi: 10.1186/s12866-015-0529-y

Fig. 3.

Fig. 3

Confocal laser scanning micrographs of 2-day P. aeruginosa PAO1 biofilms treated under different concentrations of AI-2 (×400). Bacterial viability was determined using L13152 LIVE/DEAD BacLight bacterial viability kit. a No exposure to AI-2; b Exposure to 1nM AI-2; c Exposure to 10nM AI-2. Cells staining red are considered dead while cells staining green are viable cells. The scale bar represents 20 μm