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pH dependent dynamics of HIV-1NL4-3, SIVcpzUS, SIVcpzTAN1, and HIV-2ROD 5′-leader dimerization. Each retroviral 5′-LWT, 5′-LΔAUG, and 5′-LLR constructs were incubated for 1 h in buffers containing PI salt and an indicated pH range. The monomers (M) and dimers (D) in native agarose gels are indicated. SIVcpzUS and SIVcpzTAN1 leader dimerization is promoted by the increase of pH, whereas pH with 7.0–8.0 has no significant effect on HIV-1NL4-3 and HIV-2ROD leader dimerization
