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. 2015 Sep 29;12:83. doi: 10.1186/s12977-015-0209-x

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© Tran et al. 2015

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 7 — NC binding promotes dimer formation. a Secondary structures of NC proteins for HIV-1_NL4-3, SIV_cpzUS, SIV_cpzTAN1, and HIV-2_ROD. The zinc fingers motifs (CCHC, red) are strictly conserved among selected viral strains. b–e Native agarose gel NC titration results obtained for HIV-1_NL4-3, SIV_cpzUS, SIV_cpzTAN1, and HIV-2_ROD 5′-L_WT in PI buffer. Addition of 0, 5, 10 equivalents of NC to the respective retroviral 5′-leader RNAs was probed using TB and TBM running buffers. Monomers (M) and dimers (D) are indicated