Fig. 8.

SIV_cpzTAN1 NC can promote HIV-1_NL4-3 5′-L_WT in vitro dimerization. Native agarose gel under TB and TBM running conditions were employed to detect the viral RNA leader equilibrium shift from monomer to dimer as NC was titrated at 0:1, 1:1, 5:1, 10:1 molar ratios of [NC]:[RNA]. In the first four lanes, HIV-1_NL4-3 NC was added into SIV_cpzTAN1 5′-L_WT. In the next four lanes, SIV_cpzTAN1 NC was added into HIV-1_NL4-3 5′-L_WT. In the last two lanes, HIV-1_NL4-3 NC was added into HIV-1_NL4-3 5′-L_WT. The presence or absence of protein or RNA in the ribonucleoprotein mixture is denoted as + or − sign. The monomer (M) and dimer (D) bands are marked. Note that the mobility of the HIV-1 NC-shifted SIV_cpzTAN1 5′-L_WT monomer band (at 10:1 ratio) is similar to the mobility of the NC-free dimer band