Fig. 4.

Expression patterns of Glyma11g13220 under different light conditions. All seedlings were grown under short-day conditions until 10 days after emergence (DAE), at which point half of the seedlings were transferred to long-day conditions. Fully expanded trifoliate leaves were sampled from three individual plants growing under short- and long-day conditions 12 h after dawn at 12, 15, 18, 21, 24, 27 and 30 DAE. a Image obtained approximately 53 DAE (SD, short-day conditions; LD, long-day conditions). b Quantitative real-time PCR analysis of Glyma11g13220 under short- and long-day conditions at 12, 15, 18, 21, 24, 27 and 30 DAE. Expression levels are normalized to Gmβ-tubulin (Glyma20g27280). Values are means ± SD of three biological replicates, with each measurement repeated three times. Significant differences based on the t-test are denoted by asterisks: * p < 0.05, ** p < 0.01