Figure 1. miPS-NPCs have phenotypic characteristics of immature neural progenitor cells in vitro.

(A–F) Immunocytochemical characterization of miPS-NPCs (A–C) and mE-NPCs (D–F) illustrating comparable expression of immature markers OLIG2, nestin, and proliferation marker Ki67. (G–I) No significant difference was observed following quantification of theses markers. Student’s t test was used for the statistical analysis of these experiments. (J) Semi-quantitative RT-PCR analysis, visualized using a Caliper electrophoresis, confirmed that miPS-NPCs and mE-NPCs expressed mainly transcripts associated with immature cells. Gfap mRNA expression was absent in miPS-NPCs. A–J were performed in 3 independent experiments. H, Hoechst dye. Scale bars: 100 μm.