Fig. 3.

Clinical history, and dynamics of L. pneumophila DNA load and gyrA83 mutations in respiratory samples from patient #4.

(a) The results of qPCRmip, qPCRgyrALp and NGS assays are shown, together with the antibiotic therapy for patients #4 over his hospitalization stay (see legend of Fig. 2).

(b) Dynamics of L. pneumophila DNA load, expressed as log GU/mL clinical sample, in respiratory samples of patient # 4 over time after admission in ICU. The total, reference, and mutant gyrA QRDR DNA loads are shown by circles, triangles and squares, respectively. Data were extracted from the qPCRmip assay and NGS experiments.

(c) Relative proportion of the reference (triangles) and mutant (squares) gyrA QRDR allele in respiratory samples of patient # 4 over time after admission in ICU. Data were extracted from the NGS experiments.