Table 1.
Results of the qPCRgyrALp assay with melting temperature (Tm), and presence or absence of the gyrA83/T83I QRDR change determined by PCR amplification and sequencing (PCR-Seq), and Next-Generation Sequencing (NGS, with the percentage of gyrA83 mutant alleles among sequencing reads) for the 139 respiratory samples collected from the 82 patients.
| Patients (n = 82) |
Respiratory samples (n = 139) |
Day of samplinga |
qPCRgyrALp |
T83I GyrA change |
||||
|---|---|---|---|---|---|---|---|---|
| FQ | No FQ | NAb | Total | (Tm) | PCR-Seq | NGS | ||
| n = 20 | 8 | 2 | 10 | 20 | Negative | ND | ||
| n = 58 | 3 | 0 | 1 | 4 | Negative | ND | ||
| 54 | 10c | 40 | 104 | Positive (~ 60 °C) | ND | Yes (< 0.5%) | ||
| #1 | 1 | 1 | D1 | Positive (~ 60 °C) | NPA | No | ||
| 1 | 1 | D3 | Positive (~ 56 °C) | NPA | Yes (< 0.5%) | |||
| 1 | 1 | D7 | Negative | ND | ND | |||
| #2 | 1 | 1 | D0 | Positive (~ 60 °C) | No | Yes (2.9%) | ||
| 1 | 1 | D4 | Positive (~ 56 °C) | Yes | Yes (94%) | |||
| 1 | 1 | D4 | Positive (~ 56 °C) | ND | ND | |||
| #3 | 1 | 1 | D0 | Positive (~ 56 °C) | No | No | ||
| #4 | 1 | 1 | D0 | Positive (~ 60 °C) | No | Yes (1.05%) | ||
| 1 | 1 | D2 | Positive (~ 60 °C) | ND | ND | |||
| 1 | 1 | D3 | Positive (~ 56 °C) | Yes | Yes (75%) | |||
| 1 | 1 | D5 | Positive (~ 56 °C) | Yes | Yes (85%) | |||
| Total | 75 | 13 | 51 | 139 | ||||
Data are given according to the patient's fluoroquinolone (FQ) treatment status at the time of respiratory sample collection. ND: not determined. NPA: no PCR amplification.
a
Days of sampling are given according to the day of legionellosis diagnosis (D0).
b
FQ treatment information not available.
c
Including three D0 samples tested by NGS for the three control patients.