Fig. 3.
KPT-185 does not inhibit viral production in CRISPR-Cas9 genome-edited cells expressing the resistant XPO1C258S mutant protein.
(A) Sequencing chromatogram of genomic DNA of the XPO1 region around the targeted cysteine codon from homozygous mutant XPO1C528S cells. Mutated codon (528 TGT ➔ TCA) is indicated in bold. The sgRNA sequence of the CRISPR is underlined and the PAM motif is given in magenta.
(B) Wild-type and mutant XPO1C258S HEK293T cells were transfected with HIV-1 molecular clone NL4-3 and treated with different concentrations of KPT-185. Virus production was analyzed by virus-associated p24 Gag protein in the supernatant. Error bars represent standard deviations, n = 3.