Fig. 2.

Inflammation after IRI is reduced with meclizine (100mg/kg) pretreatment 17 h prior to ischemia.

(A) Representative images of GR1 + (neutrophils) and F4/80 (macrophages) stained kidneys from sham, vehicle and meclizine pretreated mice at 48 h after IRI. Original magnification 400 ×, scale bar = 20 μm. (B and C) Quantification of GR1 + positive cells and F4/80 positive area. (D–G) Real-time PCR analysis of Il1b, Ccl2, Tnf and Il6 mRNA in sham, vehicle and meclizine pretreated mice at 48 h after IRI. Sham (n = 3), IRI + Veh and IRI + meclizine (n = 4 to 6). ***p < 0.001; **p < 0.01 and *p < 0.05. Statistical significance was determined using one-way ANOVA followed by Tukey's post-hoc test. The columns and error bars are the mean ± SEM.