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. 2015 Jul 26;4(10):653–664. doi: 10.1016/j.molmet.2015.07.004

Figure 2.

Figure 2

E4orf1-Tg mice exhibit lower body-weight gain, systemic insulin-sparing effects, inflamed and fibrotic WAT with minimal hepatic steatosis. (A) Body-weight gain (g) of male C57/Bl6 WT mice versus E4orf1-Tg mice during Dox-HFD (600 mg/kg) feeding, (n = 7 per group). (B) Glucose levels (left) and insulin levels (right) during an oral glucose tolerance test (OGTT) (2.5 g/kg body-weight glucose; single gavage) on male WT mice versus E4orf1-Tg mice, (n = 7 per group). (C) Triglyceride clearance test on WT mice versus E4orf1-Tg mice post eight-weeks Dox-HFD feeding. Mice were gavaged (20 ul/g body-weight 20% Intra-lipid) following an overnight (14–16 h fast), (n = 7 per group). (D) Ad libitum and 24 h fasted systemic glucose, TG and adiponectin levels in WT mice versus E4orf1-Tg mice post eight-weeks Dox-HFD (600 mg/kg) feeding, (n = 7 per group). (E) Representative images of H&E staining of sWAT (top left) and gWAT (middle left) tissues from WT versus E4orf1-Tg mice post eight-weeks Dox-HFD feeding. Representative images of perilipin immunohistochemical (IHC) staining of sWAT (bottom left) derived from WT versus E4orf1-Tg mice. Mac2 IHC staining (top right) and Trichrome staining (middle right) of sWAT, in addition to H&E staining of liver tissues (bottom right) from WT mice versus E4orf1-Tg mice post eight-weeks Dox-HFD feeding. Scale bar: 32 μm. (F) Fat-pad (sWAT and gWAT) and liver tissue weights (mg) normalized to body-weight (g), in WT mice versus E4orf1-Tg mice post eight-weeks Dox-HFD feeding, (n = 7 per group). (G) Hepatic TG content (mg/g) in livers derived from WT mice versus E4orf1-Tg mice post eight-weeks Dox-HFD feeding, (n = 7 per group). (Student's t-test, *P < 0.05; **P < 0.01; ***P < 0.001).