Figure 4.

Markers of dysregulated intracellular MSN signaling in the NAc of HOM wheel-runners. Representative images (left) and immunoblot analyses (right) of ΔFosB in NAc samples of (A) WT and (B) HOM rats without (sedentary) or with (runner) free access to running wheels for 5 wk (n = 5–7/group). Representative images (top) and immunoblot analyses (bottom) of (C) D1R, (D) D2R, (E) total DARPP-32 (D32), (F) pD32-(Thr³⁴), (G) pD32-(Thr⁷⁵), (H) TH, and (I) DAT in NAc samples of WT (W) and HOM (H) rats without (sedentary; SED) or with (runner; VWR) free access to running wheels for 5 wk (n = 4–7/group; data are represented relative to sedentary WT controls). Protein loading was controlled using β-tubulin or GAPDH. *p < 0.05; different letters indicate significant difference as following: ^a,bp < 0.05, genotype × treatment interaction; ^c,dp < 0.05, effect of treatment; n.s., not significant.