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. 2015 Jul 28;4(10):665–677. doi: 10.1016/j.molmet.2015.07.005

Figure 1.

Figure 1

HFD feeding decreases apoptosis of ATMs. Male C57Bl/6 mice were placed on LFD or HFD for 9 weeks. A) SVF was collected and cleaved caspase-3 was analyzed using Western blot. B) AT explants were collected and analyzed by confocal staining for the macrophage marker F4/80 (green), nuclear stain DAPI (blue), and apoptosis marker TUNEL (pink). Magnification: 40×. C–D) Quantification of TUNEL positive ATMs by percent of F4/80 positive cells (C) or by number per high-power field (D). E) Quantification of localization of apoptotic ATMs. Data are presented as mean ± SEM, A) n = 11–14/group, C–D) n = 4–8/group for confocal imaging.**p < 0.01, ***p < 0.001, ****p < 0.0001 between groups.