Table 2. Identified AAV capsid MHC class I epitopes.
| HLA allele | Epitope | Serotype | Rankpep scorea | Number of subjects reactive to capsidc | |
|---|---|---|---|---|---|
| A locus | 0101 | SADNNNSEY | AAV-2 | 63.03% | 4/7 |
| 0201/0202 | LIDQYLYYL | AAV-2 | 52.46% | 3/3 | |
| LDRLMNPLI | AAV-1 | 40.14% | 1/1 | ||
| B locus | 0702 | VPQYGYLTL | AAV-2 | 78.92% | 5/7 |
| 0801 | TTSTRTWAL | AAV-2 | 55.94% | 4/12 | |
| TTSTRTWAL | AAV-1 | 55.94% | 1/1 | ||
| 1501 | YHLNGRDSL | AAV-2 | N/Db | 2/4 | |
| 44 | SQAVGRSSF | AAV-2 | 60.24% | 6/6 | |
| 51 | VPANPSTTF | AAV-2 | 70.75% | 1/1 | |
| FPQSGVLIF | AAV-2 | 67.35% | 1/1 | ||
| 53 | QPAKKRLNF | AAV-1 | 58.02% | 1/1 | |
| N/Dd | N/Dd | YFDFNRFHCHFSPRD | AAV-2 | N/D | 2 |
| VGNSSGNWHCDSTWM | AAV-2 | N/D | 1 | ||
| QFSQAGASDIRDQSR | AAV-2 | N/D | 1 | ||
| GASDIRQSRNWLP | AAV-2 | N/D | 1 | ||
| GNRQAATADVNTQGV | AAV-2 | N/D | 1 |
N/D, not determined.
a
Percent of maximum binding affinity to the HLA allele of a consensus sequence.
b
HLA restriction determined via alternate bioinformatics algorithm (SYFPEITHI).
c
Ratio of number of subjects with positive T-cell responses to the AAV capsid over the total number of subjects tested sharing the same HLA allele.
d
No match via bioinformatics.