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. 2015 Aug 5;4(10):718–731. doi: 10.1016/j.molmet.2015.07.008

Figure 4.

Figure 4

Dual immunofluorescence of GLP-1R-eYFP expressing neurons and tyrosine hydroxylase (TH) expressing neurons throughout the brain. A. Only few TH neurons in the NTS were found to co-localise with eYFP expressing neurons. Conversely, almost all TH positive neurons in the AP co-expressed GLP-1R. B. Higher magnification image of boxed region in A. C. Around half of all TH positive neurons in the RVLM co-expressed GLP-1R, however the majority of GLP-1R neurons were TH-negative. D. A few TH positive neurons in the DMH were found to have eYFP staining, however the majority did not. E. Only a few TH positive neurons in the VTA expressed GLP-1R. Indeed the VTA contained very few GLP-1R positive somata. F–I. Scale bars in A, C, D and E = 200 μm. B, F, H and I = 20 μm. G = 50 μm. Arrow heads in B, F–I represent dual labelled neurons. * = YFP positive, TH negative neurons.