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. 2015 Aug 5;4(10):718–731. doi: 10.1016/j.molmet.2015.07.008

Figure 7.

Figure 7

GLP-1 elicits electrical responses in GLP-1R-Cre positive cells in various brain regions. A, B. Voltage-clamp recording from an RFP-positive PVN neuron. Bath application of 100 nM GLP-1 elicited a small inward current at a holding potential of −50 mV accompanied by an increase in whole-cell conductance. These effects reversed upon washout of GLP-1 from the bath solution. C, D. Similarly, in a BNST neuron bath application of 100 nM GLP-1 elicits an inward current accompanied by an increase in conductance. E. Voltage clamp recordings in RFP-positive hippocampal neurons revealed that GLP-1 either elicits an inward current (left panel) as in PVN and BNST, or an outward current. F. Mean data from current clamp recordings (left panel) demonstrating that GLP-1 causes a 10–15 mV depolarisation in most neurons tested, and a hyperpolarisation in individual hippocampal cells. Mean data from voltage clamp recordings in the right panel demonstrate the inward current of 10–20 pA amplitude elicited by 100 nM GLP-1 underlying the depolarisation in current clamp. n-numbers are given below the bars. **: p < 0.01.