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. 2014 Nov 25;6(4):e982949. doi: 10.4161/19382014.2014.982949

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© 2014 The Author(s). © 2014 Published with license by Taylor & Francis Group, LLC

© Matthew Riopel, Jinming Li, George F Fellows, Cynthia G Goodyer, and Rennian Wang

This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.

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Figure 2. — Extracellular matrix localization in the developing human fetal pancreas. (A) Scanning electron micrographs of a human fetal pancreatic cluster at 16 weeks fetal age indicating a complex organization of extracellular matrix (ECM) on the cell surface. Highlighted areas are enlarged in (A1) and (A2). Scale bars = 10 μm (A and A₁) and 1 μm (A₂). (B) Transmission electron micrograph of ECM-endocrine cell interactions in the human fetal pancreas (8 and 14 weeks fetal age). Endo = endocrine cells, scale bars = 1 μm. (C) Double immunofluorescence staining of a 14 week fetal age pancreas for insulin (red) with collagen I, collagen IV, fibronectin or laminin (all green), and DAPI-stained nuclei in blue. Scale bar = 50 μm, asterisks = blood cells. Representative images from 4–10 pancreata are shown.